Part:BBa_K5371101
ERG20-(GGGS)3-MRBBS
The ERG20-(GGGS)3-MRBBS assembly is a composite part for the effective production of α-biasobolol in Saccharomyces cerevisiae. It is a combination of CDS ERG20 Part:BBa_K849001 and MRBBS via a flexible linker (GGGS)3. The ERG20 expresses farnesyl pyrophosphate synthetase, which catalyzes the biosynthesis of farnesyl pyrophosphate (FPP). The MRBBS expresses α-bisabolol synthase, which catalyzes FPP into α-biasobol. The (GGGS)3 is a flexible linker tested and selected from various linker sequences, which was proved to improve the production of α-biasobolol when fusing ERG20 and MRBBS. The ERG20-(GGGS)3-MRBBS was fused with a GAL10 promoter upstream, which triggers the production of α-biasobolol at the presence of GAL4 protein. It may be regulated by other kinds of promoters for other experimental purposes. To optimize the production of α-biasobolol, the original ERG9, which catalyzes FPP into squalene, should be knocked down. However, because ERG9 is essential for the viability of Saccharomyces cerevisiae, it should not be completely knocked out.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1680
Illegal SpeI site found at 2372 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1680
Illegal SpeI site found at 2372 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1680
Illegal BglII site found at 2406 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1680
Illegal SpeI site found at 2372 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1680
Illegal SpeI site found at 2372 - 1000COMPATIBLE WITH RFC[1000]
//cds/enzyme
//function/biosynthesis
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